T-Track® IFN-γ HiSpecificityPRO
Size: Includes 96-well strip plate
Price: 220.00 €
Delivery time: Approximately 2-7 days; please note: solid plate format (12200002) currently not available.
Not available in: No restrictions. Please note that the prices of our products do not include shipping and freight costs, charges, taxes or customs duties.
T-Track® human IFN-γ ELISpot kit HiSpecificityPRO
The all-in-one and ready-to-use ELISpot kit was developed to obtain improved results for the assessment of interferon gamma (IFN-γ) release. The ELISpot assay consequently evaluates antigen-specific cellular activity at a single-cell level and therefore enables enumeration of IFN-γ-producing cells.
The T-Track® human IFN-γ ELISpot kit HiSpecificityPRO facilitates fast and standardized read-outs, combined with highest specificity and lowest background*.
T-Track® IFN-γ HiSpecificityPRO components
- Precoated PVDF microtiter plate
- Positive control (PHA)
- Enzyme (ALP)-conjugated detection mAb
- All needed buffers
- Instructions for Use
- All-in-one and ready-to-use ELISpot kit
- One-step detection system
- Compatible with Lophius' T-activated® proteins
- Highest specificity*
- Low background
- Homogeneous spot morphology
- Standardized read-out
*compared to competitor human IFN-γ ELISpot kits
The ELISpot is a common method for monitoring cellular immune responses to antigen stimulation. The general setup consists of a 96-well microtiter plate (strip or solid format), with a PVDF membrane at the bottom of each well. A capture antibody is immobilized onto the membrane to bind cytokines secreted by the antigen-stimulated cells. After applying an enzyme-conjugated detection antibody and following an enzymatic staining reaction, spots are revealed whereby one spot represents the unique cellular activity of antigen-specific cytokine-secreting cells at a single-cell level.
Comparative analysis of IFN-γ ELISpot kits
The T-Track® ELISpot kit human IFN-γ HiSpecificityPRO was compared to a competitor’s human IFN-γ ELISpot kit to measure T cell-mediated release of IFN-γ at a single-cell level (Figures 1-2). Per well, 100,000 freshly-prepared peripheral blood mononuclear cells (PBMCs) from three CMV-seronegative and three CMV-seropositive donors were used. For comparative analysis, stimulation of PBMCs was performed with JPT PepMix™ peptide pool pp65 for 20 h at 37°C and 5% CO2. Experimental procedures were performed according to the respective manufacturer‘s protocol. IFN-γ release was determined either in a one-step (Lophius) or a two-step detection system (Competitor).
In contrast to the competitor’s ELISpot kit (Figure 1D), no background spot was detected following pp65 stimulation of PBMCs from CMV-seronegative donors using Lophius’ ELISpot kit (Figure 1C), limiting the risk of false-positive results. Beside a reduced background and increased specificity, results obtained by the Lophius ELISpot kit are characterized by the detection of discrete, homogeneous spots from pp65-stimulated PBMCs from CMV-seropositive donors (Figure 1A), allowing accurate spot enumeration, unlike the competitor’s ELISpot read-out (Figure 1B).
Lophius’ IFN-γ HiSpecificityPRO ELISpot kit offers a highly specific and reliable assay with very low spot counts in unstimulated PBMCs from various CMV- and CMV+ donors (Figure 2A, green bars), as opposed to its competitor’s IFN-γ ELISpot kit (Figure 2A, grey bars). Following pp65 stimulation (Figure 2B), Lophius’ IFN-γ HiSpecificityPRO ELISpot kit demonstrated higher specificity and comparable sensitivity (green bars) over its competitor kit (grey bars).
T-Track® Human IFN-y ELISpot kit HispecificityPRO
ImevaX develops novel vaccination strategies against globally relevant pathogens, specifically Helicobacter pylori infections.
For our clinical studies we use Lophius’ human IFN-γ ELISpot kits. This time-saving kit provides all reagents needed to perform a standardized assay, with reduced background, homogeneous spots and above all, reproducible results.
To get fast, accurate and reliable data, we also use Lophius' convenient ELISpot read-out service, as an efficient alternative to buying an ELISpot reader.
Dr. Kathrin Klar
ImevaX GmbH - Munich
Lophius' IFN-γ Products
Working with Lophius’ pre-coated IFN-γ ELISpot plates provides the flexibility to start ELISpot assays as soon as patient and research samples become available, and saves our laboratory staff the time normally used for self-coating.
Fulfilling highest quality standards, Lophius’ ELISpot plates yield low background and specific homogeneous spots, supporting a standardized and reproducible read-out compared to self-coated ELISpot plates.
Therefore, this convenient solution makes our daily work, both in antiviral immune-therapeutics research and in routine in-house and external diagnostics, more effective and reliable.
Prof. Dr. rer. nat. Britta Eiz-Vesper