Reverse T Cell Technology (RTT)

RTT represents a novel strategy to distinguish between antigen-specific in vivo-activated and memory T cells, allowing the discrimination between an active and latent stage of a disease, respectively. 

Fields of application:

  • Differential diagnosis of tuberculosis (TB), distinguishing between active disease and latent infection
  • Diagnosis of disease status and monitoring of therapy success for autoimmune diseases that depend on the T cell status (e.g. relapsing vs. remitting multiple sclerosis)  
  • Analysis of T cell responses following vaccination protocols

Reverse T Cell Technology (RTT):
Discrimination between in vivo-activated and memory T cells  

T cells control and orchestrate the immune system, thus playing a key role in immunological defence mechanisms against pathogens or in autoimmune diseases. In vivo-activated T cells are known to transiently appear in the peripheral blood during active microbial infection or chronic inflammatory disease and thus constitute an ideal immunological marker for the reliable detection of an active infection or the early diagnosis of autoimmune disease, respectively. The Lophius RTT detects two different markers for a differentiated diagnosis.

Induced by the antigen-specific contact between an APC and the in vivo-activated T (helper) cell, a maturation process in the APC is initiated which generates the activation marker (Figure 3). On the other hand, the memory marker is produced by memory T cells which become reactivated by the APC presenting the specific antigen (Figure 4).

With the Lophius proprietary RTT a differentiated diagnosis of tuberculosis is possible, closing a major gap in the current TB diagnostic landscape, as IGRAs (Interferon Gamma Release Assays) cannot distinguish between active disease and latent infection.

 

 

Figure 3 – In vivo-activated T cells provide a maturation signal to the APC and induce expression of a biological marker (activation marker). This constitutes an ideal immunological marker for the reliable detection of an active infection or the early diagnosis of an autoimmune disease
Figure 4 – Detection of memory marker synthesized by memory T cell after reactivation by APC.

Advantages of RTT:

  • Novel strategy to distinguish between antigen-specific in vivo-activated Th cells and memory T cells
  • Applicable to multiple read-out systems  

Fields of application:

  • Differential diagnosis of tuberculosis (TB), distinguishing between active disease and latent infection
  • Diagnosis of the disease status and monitoring of therapy efficacy for autoimmune diseases in which activated T cells are involved in the pathogenesis of the disease (e.g. multiple sclerosis, diabetes)
  • Analysis of T cell responses following vaccination protocols  

References:

  • den Haan JMM, Bevan MJ (2000). A novel helper role for CD4 T cells. Proc. Natl. Acad. Sci. USA 97:12950-12952. (Read more)

International patent:

  • WO/2012/037937  “METHOD FOR DETECTING, DIFFERENTIATING, AND QUANTIFYING T CELL POPULATIONS BY MEANS OF REVERSE TRANSCRIPTION QUANTITATIVE REAL-TIME PCR (RT-QPCR) TECHNOLOGY”

 

 

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